Bottom-up physiologically-based biokinetic modelling as an alternative to animal testing

Human skin equivalent as an alternative to animal testing

The 3-D skin equivalent can be viewed as physiologically comparable to the natural skin and therefore is a suitable alternative for animal testing. This highly differentiated in vitro human skin equivalent is used to assess the efficacy and mode of action of novel agents. This model is generated from primary human keratinocytes on a collagen substrate containing human dermal fibroblasts. It is ...

set-up an alamarblue based viability assay as an alternative method to routine mtt tests

Use of Physiologically Based Biokinetic (PBBK) Modeling to Study Estragole Bioactivation and Detoxification in Humans as Compared with Male Rats

The extent of bioactivation of the herbal constituent estragole to its ultimate carcinogenic metabolite 1'-sulfooxyestragole depends on the relative levels of bioactivation and detoxification pathways. The present study investigated the kinetics of the metabolic reactions of both estragole and its proximate carcinogenic metabolite 1'-hydroxyestragole in humans in incubations with relevant tissu...

Fear Response in Roosters Orally Exposed to Alcohol: An Alternative Animal Model

Effect of ethanol consumption on fear response in human and conventional animal models (rodents) is controversial. The present study aimed to investigate the effect of ethanol administration on fearfulness in poultry as an alternative animal model.Being a reliable measure in avian species, tonic immobility was used to evaluate the fear response in 60 roosters orally administered with ethanol. D...

Confidence Intervals as an Alternative to Significance Testing

The article argues to replace null hypothesis significance testing by confidence intervals. Correctly interpreted, confidence intervals avoid the problems associated with null hypothesis statistical testing. Confidence intervals are formally valid, do not depend on apriori hypotheses and do not result in trivial knowledge. The first part presents critique of null hypothesis significance testing...